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Permeability Of Hydrophilic
SUMMARY
Investigations of the integrity and transport characteristics of 2/4/A1 cells
have been done in this report. The cell line was isolated from rat fetal
intestinal epithelial cells and transfected with thermolabile SV40 large T
antigen.
These cells proliferated at 33 °C, but eliminated the antigen and ceased
proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells
started to differentiate but simultaneously the cells also underwent massive
cell death.
When cultured at 37°C these cells formed confluent and tight monolayers that
seemed to have paracellular transport characteristics similar to that of th ....
Word count: 2422 - Page count: 9
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